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Our Abpromise guarantee covers the use of ab2918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Can be blocked with FKBP12 peptide (ab4935).|
|IHC-Fr||1/700. PubMed: 16171515|
This image is courtesy of an Abreview submitted by Dr sudhish mishra
ab2918 at 1/1000 staining FKBP12 from human penis tissue by IHC-P. The tissue was paraformaldehyde fixed and the slides were incubated in citrate buffer for antigen retrieval and heated for 20 minutes. The tissue was incubated with ab2918 for 24 hours. The secondary antibody used was part of the DAKO ENVISON System. The picture depicts staining of nerve fibers within corporal tissue of the human penis.
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