May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Belongs to the FKBP-type PPIase family. FKBP1 subfamily. Contains 1 PPIase FKBP-type domain.
Western blot - Anti-FKBP12 antibody [EP3887] (ab108420)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg) Lane 3: SH-SY5Y whole cell lysate (20 µg) Lane 4: Human brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108420 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108420 was shown to recognize FKBP12 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP12 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab108420 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - FKBP12 antibody [EP3887] (ab108420)
All lanes : Anti-FKBP12 antibody [EP3887] (ab108420) at 1/1000 dilution
Lane 1 : Fetal brain lysate Lane 2 : HL-60 brain lysate Lane 3 : U937 lysate Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution