Anti-FKBP12 antibody [EPR3888] (ab92459)

Overview

  • Product nameAnti-FKBP12 antibody [EPR3888]
    See all FKBP12 primary antibodies
  • Description
    Rabbit monoclonal [EPR3888] to FKBP12
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues at the C terminus of Human FKBP12.

  • Positive control
    • Fetal brain, U937 and SH-SY5Y cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR3888
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab92459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notesICC: 1/50 - 1/100.
    WB: 1/10,000 - 1/50,000. Predicted molecular weight: 12 kDa.

    Is unsuitable for Flow Cyt, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
    • Sequence similaritiesBelongs to the FKBP-type PPIase family. FKBP1 subfamily.
      Contains 1 PPIase FKBP-type domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12 kDa FK506-binding protein antibody
      • 12 kDa FKBP antibody
      • Calstabin 1 antibody
      • FK506 binding protein 1 antibody
      • FK506 binding protein 12 antibody
      • FK506 binding protein 1A (12kD) antibody
      • FK506 binding protein 1A 12kDa antibody
      • FK506 binding protein 1A antibody
      • FK506 binding protein T cell 12 kD antibody
      • FK506 binding protein, T cell, 12 kD antibody
      • FK506 binding protein12 antibody
      • FK506-binding protein 1A antibody
      • FKB1A_HUMAN antibody
      • FKBP 12 antibody
      • FKBP 1A antibody
      • FKBP-12 antibody
      • FKBP-1A antibody
      • FKBP1 antibody
      • FKBP12 antibody
      • FKBP12 Exip3 antibody
      • FKBP12C antibody
      • fkbp1a antibody
      • Immunophilin FKBP12 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
      • PKC12 antibody
      • PKCI2 antibody
      • PPIase antibody
      • PPIase FKBP1A antibody
      • Protein kinase C inhibitor 2 antibody
      • Rotamase antibody
      see all

    Anti-FKBP12 antibody [EPR3888] images

    • All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : U937 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 12 kDa

    References for Anti-FKBP12 antibody [EPR3888] (ab92459)

    ab92459 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (cultured cell)
    Gel Running Conditions Reduced Denaturing (4%-12% bis tris)
    Loading amount 30 µg
    Specification cultured cell
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 09 2016

    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (Exponential growth)
    Gel Running Conditions Reduced Denaturing (4-15%)
    Loading amount 10 µg
    Specification Exponential growth
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 09 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"