Overview

  • Product nameAnti-FKBP135 antibody
    See all FKBP135 primary antibodies
  • Description
    Rabbit polyclonal to FKBP135
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human FKBP135 aa 50-100.

  • Positive control
    • HeLa cells nuclear extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibody was purified using an epitope specific to FKBP-135 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200.
WB 1/2000 - 1/10000. Predicted molecular weight: 135 kDa.
IP Use a concentration of 1 - 4 mg/ml.

Target

  • FunctionMay be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.
  • Sequence similaritiesBelongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • DomainThe PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
    The central coiled-coil region is responsible for association with early endosomes.
  • Cellular localizationCytoplasm. Cell projection > axon. Early endosome. Present in axons and neuronal growth cones.
  • Information by UniProt
  • Database links
  • Alternative names
    • 133 kDa FK506-binding protein antibody
    • 133 kDa FKBP antibody
    • C430014M02Rik antibody
    • FK506 binding protein 135 antibody
    • FK506-binding protein 15 antibody
    • FKB15_HUMAN antibody
    • FKBO133 antibody
    • FKBP-133 antibody
    • FKBP-15 antibody
    • FKBP133 antibody
    • Fkbp15 antibody
    • KIAA0674 antibody
    • mKIAA0674 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • RIKEN cDNA C430014M02 gene antibody
    • RP23 64F17.7 antibody
    • WAFL antibody
    • WASP and FKBP-like antibody
    see all

Anti-FKBP135 antibody images

  • ab14432 staining FKBP135 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. An Alexa Fluor®597-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Cells labelled for FKBP15 displayed punctate staining, which was absent in cells treated with siRNA against FKBP15.

    See Abreview

  • All lanes : Anti-FKBP135 antibody (ab14432) at 0.2 µg/ml

    Lane 1 : Nuclear lysates prepared from Hela cells at 5 µg
    Lane 2 : Nuclear lysates prepared from Hela cells at 20 µg
    Lane 3 : Nuclear lysates prepared from Hela cells at 1 mg/ml
    Lane 4 : Nuclear lysates prepared from Hela cells at 1 mg/ml
    Lane 5 : Nuclear lysates prepared from Hela cells at 1 mg/ml


    Predicted band size : 135 kDa

    Antibodies: ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection: Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).

    In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2µg/mg of extract.

  • ab14432 at 1/1000 staining mouse liver tissue sections by IHC-P. The tissue sections were paraformaldehyde fixed and blocked prior to incubation with the ab14432 for 24 hours. An HRP conjugated secondary antibody was used. The image shows staining in liver cells of a female mouse at 20X magnification.

    See Abreview

References for Anti-FKBP135 antibody (ab14432)

This product has been referenced in:
  • Zavodszky E  et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). ICC/IF ; Human . Read more (PubMed: 24819384) »
  • Viklund IM  et al. Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel disease: a potential marker gene for ulcerative colitis. Int J Colorectal Dis 23:921-30 (2008). IHC (PFA fixed) ; Human . Read more (PubMed: 18654788) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.1% Triton TX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2013

Thank you for contacting us and your interest in our products.


I am sorry if it was misleading but the image supplied with Anti-FKBP135 antibodies ab14432, ab14433 and ab14434 are the same. They illustrate the IP performed with ab14432,...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Miss. gwen Lagoda

Verified customer

Submitted Apr 27 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Penis Sections)
Specification Penis Sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Miss. gwen Lagoda

Verified customer

Submitted Apr 12 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Penis)
Specification Human Penis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100
Username

Miss. gwen Lagoda

Verified customer

Submitted Feb 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"