Overview

  • Product name
  • Description
    Rabbit polyclonal to FKBP25
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, African green monkey
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FKBP25.

    (Peptide available as ab16979.)

  • Positive control
    • HeLa nuclear extracts HeLa Whole Cell Lysate HeLa Nuclear Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/1000.
WB Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
  • Sequence similarities
    Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 25 kDa FK506-binding protein antibody
    • 25 kDa FKBP antibody
    • FK506 binding protein 25 T cell antibody
    • FK506 binding protein 25, T cell, 25-KD antibody
    • FK506 binding protein 3 (25kD) antibody
    • FK506-binding protein 3 antibody
    • FKBP 25 antibody
    • FKBP 3 antibody
    • FKBP-25 antibody
    • FKBP-3 antibody
    • FKBP25 antibody
    • FKBP3 antibody
    • FKBP3_HUMAN antibody
    • Immunophilin FKBP25 antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP3 antibody
    • PPIase antibody
    • PPIase FKBP3 antibody
    • Rapamycin binding protein antibody
    • Rapamycin-selective 25 kDa immunophilin antibody
    • Rotamase antibody
    see all

Anti-FKBP25 antibody images

  • All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : HeLa Nuclear Lysate
    Lane 3 : HeLa Whole Cell Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml
    Lane 4 : HeLa Nuclear Lysate with Human FKBP25 peptide (ab16979) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FKBP25 antibody (ab16654) at 0.05 µg/ml

    Lane 1 : HeLa nuclear extracts
    Lane 2 : HeLa nuclear extracts with Human FKBP25 peptide (ab16979) at 1 µg/ml
    Lane 3 : HeLa nuclear extracts with Human FKBP25 peptide (ab16980) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 25 kDa

    This image is courtesy of collaborator data

    ab16654 specifically recognises FKBP25 (lane1), which can be blocked using the N-terminal immunizing peptide (lane2) but not the corresponding C-terminal peptide (lane3).
  • ab16654 immunoprecipitating FKBP25 from rat PC12 whole cell lysate. A protein A matrix was used and the antibody was used at 4ug/mg of the lysate. Ab16654 was used in subsequent western blot (Lane 1). Lane 2 shows control IgG immunoprecipitate, Lane 3 shows input material.

    See Abreview

  • All lanes : Anti-FKBP25 antibody (ab16654) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
  • Image courtesy of Human Protein Atlas

    ab16654 staining FKBP25 in human testis, showing a distinct and strong staining pattern at cells in ductus seminiferus. Paraffin embedded human skin tissue was incubated with ab16654 (1/1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16654 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

References for Anti-FKBP25 antibody (ab16654)

This product has been referenced in:
  • Ochocka AM  et al. FKBP25, a novel regulator of the p53 pathway, induces the degradation of MDM2 and activation of p53. FEBS Lett 583:621-6 (2009). WB ; Human . Read more (PubMed: 19166840) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
500 µg
Specification
293T
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Oct 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12)
Total protein in input
500 µg
Specification
PC12
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Oct 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
No blocking step used for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
20 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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