Overview

  • Product name
  • Description
    Rabbit polyclonal to FKBP51
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human, Xenopus laevis, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human FKBP51 aa 394-407.
    Sequence:

    CQKKAKEHNERDRR


    Database link: Q13451
    (Peptide available as ab4924)

  • Positive control
    • WB: HeLa cell lysate. K562, HepG2, PC-3 and MCF7 nuclear enriched extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab2901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa.Can be blocked with FKBP51 peptide (ab4924). By Western blot, this antibody detects an ~51 kDa protein representing FKBP 51 in HeLa cell lysate. By Western blot this antibody also detects FKBP 51 in whole tissue extracts from rat kidney and rat and mouse testes.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20422297

Target

  • Function
    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • Tissue specificity
    Widely expressed, enriched in testis compared to other tissues.
  • Sequence similarities
    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 51 kDa FK506 binding protein 5 antibody
    • 51 kDa FK506 binding protein antibody
    • 51 kDa FK506-binding protein antibody
    • 51 kDa FKBP antibody
    • 54 kDa progesterone receptor associated immunophilin antibody
    • 54 kDa progesterone receptor-associated immunophilin antibody
    • AIG 6 antibody
    • AIG6 antibody
    • Androgen regulated protein 6 antibody
    • Androgen-regulated protein 6 antibody
    • FF1 antigen antibody
    • FK506 binding protein 5 antibody
    • FK506-binding protein 5 antibody
    • FKBP 5 antibody
    • FKBP 51 antibody
    • FKBP 54 antibody
    • FKBP-5 antibody
    • FKBP-51 antibody
    • FKBP5 antibody
    • FKBP5_HUMAN antibody
    • FKBP54 antibody
    • HSP90 binding immunophilin antibody
    • HSP90-binding immunophilin antibody
    • MGC111006 antibody
    • OTTHUMP00000016268 antibody
    • P54 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP5 antibody
    • Ptg 10 antibody
    • Ptg10 antibody
    • Rotamase antibody
    • T cell FK506 binding protein antibody
    see all

Images

  • All lanes : Anti-FKBP51 antibody (ab2901) at 2 µg/ml

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear enriched extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) nuclear enriched extract
    Lane 3 : PC-3 (human prostate adenocarcinoma cell line) nuclear enriched extract
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) nuclear enriched extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-Rabbit IgG (H+L) at 1/2500 dilution

    Approximately 51 and 45 kDa bands corresponding to FKBP51 were observed across cell lines tested

  • Anti-FKBP51 antibody (ab2901) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

References

This product has been referenced in:
  • Li K  et al. ELF5-Mediated AR Activation Regulates Prostate Cancer Progression. Sci Rep 7:42759 (2017). Read more (PubMed: 28287091) »
  • Allan AM  et al. Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning. PLoS One 9:e96200 (2014). WB ; Mouse . Read more (PubMed: 24755652) »

See all 9 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293Tcells)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
20 µg
Specification
293Tcells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Specification
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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