Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab126715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

  • Function
    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • Tissue specificity
    Widely expressed, enriched in testis compared to other tissues.
  • Sequence similarities
    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 51 kDa FK506 binding protein 5 antibody
    • 51 kDa FK506 binding protein antibody
    • 51 kDa FK506-binding protein antibody
    • 51 kDa FKBP antibody
    • 54 kDa progesterone receptor associated immunophilin antibody
    • 54 kDa progesterone receptor-associated immunophilin antibody
    • AIG 6 antibody
    • AIG6 antibody
    • Androgen regulated protein 6 antibody
    • Androgen-regulated protein 6 antibody
    • FF1 antigen antibody
    • FK506 binding protein 5 antibody
    • FK506-binding protein 5 antibody
    • FKBP 5 antibody
    • FKBP 51 antibody
    • FKBP 54 antibody
    • FKBP-5 antibody
    • FKBP-51 antibody
    • FKBP5 antibody
    • FKBP5_HUMAN antibody
    • FKBP54 antibody
    • HSP90 binding immunophilin antibody
    • HSP90-binding immunophilin antibody
    • MGC111006 antibody
    • OTTHUMP00000016268 antibody
    • P54 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP5 antibody
    • Ptg 10 antibody
    • Ptg10 antibody
    • Rotamase antibody
    • T cell FK506 binding protein antibody
    see all

Images

  • Predicted band size: 51 kDa



    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126715 observed at 51 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab126715 was shown to recognize FKBP51 in wild-type cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. Ab126715 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-FKBP51 antibody [EPR6617] (ab126715) at 1/1000 dilution

    Lane 1 : Human testis cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 51 kDa

  • ab126715, at 1/50 dilution, stains FKBP51 in paraffin embedded human colon tissue by immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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