Overview

  • Product name
    Anti-FKBP51 antibody [Hi51B]
    See all FKBP51 primary antibodies
  • Description
    Mouse monoclonal [Hi51B] to FKBP51
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Dog, Human
  • Immunogen

    Full length Human FKBP51, binding epitope in TPR domain

  • Positive control
    • HeLa cell lysate. Whole tissue extracts from rat kidney and rat and mouse testes.

Properties

Applications

Our Abpromise guarantee covers the use of ab79844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/2000. Predicted molecular weight: 51 kDa.

Target

  • Function
    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • Tissue specificity
    Widely expressed, enriched in testis compared to other tissues.
  • Sequence similarities
    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 51 kDa FK506 binding protein 5 antibody
    • 51 kDa FK506 binding protein antibody
    • 51 kDa FK506-binding protein antibody
    • 51 kDa FKBP antibody
    • 54 kDa progesterone receptor associated immunophilin antibody
    • 54 kDa progesterone receptor-associated immunophilin antibody
    • AIG 6 antibody
    • AIG6 antibody
    • Androgen regulated protein 6 antibody
    • Androgen-regulated protein 6 antibody
    • FF1 antigen antibody
    • FK506 binding protein 5 antibody
    • FK506-binding protein 5 antibody
    • FKBP 5 antibody
    • FKBP 51 antibody
    • FKBP 54 antibody
    • FKBP-5 antibody
    • FKBP-51 antibody
    • FKBP5 antibody
    • FKBP5_HUMAN antibody
    • FKBP54 antibody
    • HSP90 binding immunophilin antibody
    • HSP90-binding immunophilin antibody
    • MGC111006 antibody
    • OTTHUMP00000016268 antibody
    • P54 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP5 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP5 antibody
    • Ptg 10 antibody
    • Ptg10 antibody
    • Rotamase antibody
    • T cell FK506 binding protein antibody
    see all

Images



  • Predicted band size : 51 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab79844 observed at 51 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab79844 was shown to recognize FKBP51 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. Ab79844 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ab79844 at 1/100 dilution staining FKBP51 in normal MK cells (IF localization shown in red) by Immunocytochemistry/ immunofluorescence.

References

ab79844 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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