The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may optmize staining.
Note: expected to work on frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionImmunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
DomainThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA). The C-terminal region (AA 375-458) is required to prevent tubulin polymerization. The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
Post-translational modificationsPhosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue, using 1/100 ab84536 in conjunction with 1/100 red-fluorescent Goat anti-Rabbit IgG highly cross-adsorbed antibody Hilyte PlusTM 555.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody (ab84536)Image from Wang N et al., PLoS One. 2012;7(3):e34010. Epub 2012 Mar 20. Fig 6.; doi:10.1371/journal.pone.0034010; March 20, 2012, PLoS ONE 7(3): e34010.
Immunohistochemical analysis of Human endometrial tissue, staining FKBP52 with ab84536 at 1/300 dilution. Staining was detected using DAB.
References for Anti-FKBP52 antibody (ab84536)
This product has been referenced in:
Wang N et al. Expression of PRB, FKBP52 and HB-EGF relating with ultrasonic evaluation of endometrial receptivity. PLoS One7:e34010 (2012).
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