Overview

  • Product nameAnti-FKBP52 antibody
    See all FKBP52 primary antibodies
  • Description
    Rabbit polyclonal to FKBP52
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-177 of Human FKBP52 (AAH01786)

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells. Cal27 xenograft.

Applications

Our Abpromise guarantee covers the use of ab97306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionImmunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • DomainThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • Post-translational
    modifications
    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 51 kDa FK506-binding protein antibody
    • 52 kDa FK506 binding protein antibody
    • 52 kDa FK506-binding protein antibody
    • 52 kDa FKBP antibody
    • 59 kDa immunophilin antibody
    • FK506 binding protein 4 antibody
    • FK506-binding protein 4 antibody
    • FKBP 4 antibody
    • FKBP 52 antibody
    • FKBP 59 antibody
    • FKBP-4 antibody
    • FKBP-52 antibody
    • FKBP4 antibody
    • FKBP4_HUMAN antibody
    • FKBP51 antibody
    • FKBP52 protein antibody
    • FKBP59 antibody
    • HBI antibody
    • Hsp 56 antibody
    • HSP binding immunophilin antibody
    • HSP-binding immunophilin antibody
    • Hsp56 antibody
    • Immunophilin FKBP52 antibody
    • N-terminally processed antibody
    • p52 antibody
    • p59 antibody
    • p59 protein antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
    • Peptidylprolyl cis trans isomerase antibody
    • PPIase antibody
    • PPIase FKBP4 antibody
    • Rotamase antibody
    • T cell FK506 binding protein 59kD antibody
    see all

Anti-FKBP52 antibody images

  • Anti-FKBP52 antibody (ab97306) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab97306 at 1/200 dilution (1) and merged with DNA probe (2).
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab97306 at 1/500 dilution.
  • Anti-FKBP52 antibody (ab97306) at 1/3000 dilution + Recombinant human FKBP52 protein (ab84189) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Anti-FKBP52 antibody (ab97306)

This product has been referenced in:
  • Martins RP  et al. Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection. J Proteomics 75:4457-70 (2012). WB ; Pig . Read more (PubMed: 22507201) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (Intestinal mucosa)
Loading amount 30 µg
Specification Intestinal mucosa
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Melania Collado-Romero

Verified customer

Submitted Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"