Anti-Flagellin antibody (ab106146)

Overview

  • Product nameAnti-Flagellin antibody
    See all Flagellin primary antibodies
  • Description
    Rabbit polyclonal to Flagellin
  • Specificityab106146 is specific for Lyme Borrelia spp Flagellin protein. Cross reactivity is expected with Borrelia burgdorferi, garinii and valaisiana sources of Flagellin protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab106146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 76.3 kDa.
ELISA 1/6000.

Target

  • RelevanceFlagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs).
  • Cellular localizationSecreted. Bacterial flagellum.
  • Alternative names
    • 41 kDa antigen antibody
    • b1923 antibody
    • flaF antibody
    • Flagellar filament 41 kDa core protein antibody
    • Flagellin antibody
    • fliC antibody
    • hag antibody
    • JW1908 antibody
    • P41 antibody
    see all

Anti-Flagellin antibody images

  • All lanes : Anti-Flagellin antibody (ab106146) at 1/1000 dilution

    Lane 1 : MBP-Flagellin fusion protein (the immunogen)
    Lane 2 : MBP alone

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat anti-rabbit IgG at 1/40000 dilution

    Predicted band size : 76.3 kDa

References for Anti-Flagellin antibody (ab106146)

ab106146 has not yet been referenced specifically in any publications.

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Based on the sequences available on UniProt, I don't expect any of our flagellin antibodies to react with pseudomonas samples. There were multiple pu...

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