Overview

  • Product nameAnti-Flagellin antibody
    See all Flagellin primary antibodies
  • Description
    Rabbit polyclonal to Flagellin
  • SpecificityThis antibody is specific for bacterial Flagellin (FliC).
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli , Salmonella anatum, Salmonella selandia
  • Immunogen

    Purified recombinant Flagellin made in BL21 bacteria.

  • Positive control
    • Lysates transfected with purified flagellin, E. Coli total lysate or 293T transfected with recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab93713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 60 kDa.
ICC/IF 1/100.

Target

  • RelevanceFlagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs).
  • Cellular localizationSecreted. Bacterial flagellum.
  • Database links
    • Alternative names
      • 41 kDa antigen antibody
      • b1923 antibody
      • flaF antibody
      • Flagellar filament 41 kDa core protein antibody
      • Flagellin antibody
      • fliC antibody
      • hag antibody
      • JW1908 antibody
      • P41 antibody
      see all

    Anti-Flagellin antibody images

    • All lanes : Anti-Flagellin antibody (ab93713) at 1/10000 dilution

      Lane 1 : 293T transfected with recombinant protein
      Lane 2 : 293T transfected with recombinant protein


      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.

      Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.
    • Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.

      Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.

    References for Anti-Flagellin antibody (ab93713)

    This product has been referenced in:
    • Evans KL  et al. Eliminating a set of four penicillin binding proteins triggers the Rcs phosphorelay and Cpx stress responses in Escherichia coli. J Bacteriol 195:4415-24 (2013). Read more (PubMed: 23893115) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    Unfortunately, I do not know which bacteria you are investigating. Ifyou are looking at peritrichous bacteria such as E.coli or salmonella, the flagella are all over the bacteria, so the antibody is going to light...

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    Thank you for contacting us.

    The flagellin antibody was generated against recombinant purified
    full-length FliC from E Coli DH5a. There are similarities at the N and C
    termini with P aeruginosa FliC but it is probably not enough fo...

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    Thank you for contacting us and thank you for your patience.

    The protocol seems fairly standard. However, she was in contact with the originator of this antibody, and here aretheir thoughts:

    1) The rods are certainly full of newly e...

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    Thank you for your call earlier and for your patience while I have looked into your enquiry.

    Based on the sequences available on UniProt, I don't expect any of our flagellin antibodies to react with pseudomonas samples. There were multiple pu...

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    Thank you for contacting Abcam. The concentration of protein in ab93713 is between 30 and 40 mg/ml. We do not know how much of that total is made up with antibody because the sample is serum, not purified antibody. Please let me know if there is anythi...

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    Thank you for contacting us. The total protein concentration of ab93713, lot GR35089-1, is around 30-40 mg/ml. However, since this is whole serum containing a wide variety of proteins, the concentration of the specific anti-Flagellin antibody canno...

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    J'ai été informé par laboratoire que l'anticorps ab93713 peut en effet détecter la flagelline de Salmonella typhimurium. Malheureusement ils n'ont jamais testé la réactivité de l'anticorps avec bacilis subtilis. Comme expliqué par téléphone, nous n’off...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"