Overview

  • Product name
  • Description
    Goat polyclonal to FLAP
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    C-KTISTTISPLLLIP

    , corresponding to C terminal amino acids 148-161 of Human FLAP

  • Positive control
    • Human brain and U937 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab39535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 48 kDa, (predicted molecular weight: 19 kDa). An explanation has not been found for the difference in the two molecular weight values.
    This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
    • Involvement in disease
      Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
      Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition.
    • Sequence similarities
      Belongs to the MAPEG family.
    • Domain
      The C-terminal part after residue 140 is mostly unstructured.
    • Cellular localization
      Nucleus membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • 5-lipoxygenase activating protein antibody
        • AL5AP_HUMAN antibody
        • ALOX 5AP antibody
        • ALOX5 AP antibody
        • ALOX5AP antibody
        • Arachidonate 5 lipoxygenase activating protein antibody
        • Arachidonate 5-lipoxygenase-activating protein antibody
        • Five lipoxygenase activating protein antibody
        • FLAP antibody
        • MK 886 binding protein antibody
        • MK-886-binding protein antibody
        see all

      References

      This product has been referenced in:
      • Sharma-Walia N  et al. The Kaposi's sarcoma-associated herpesvirus (KSHV)-induced 5-lipoxygenase-leukotriene B4 cascade plays key roles in KSHV latency, monocyte recruitment, and lipogenesis. J Virol 88:2131-56 (2014). Read more (PubMed: 24335295) »
      • Maier TJ  et al. Celecoxib inhibits 5-lipoxygenase. Biochem Pharmacol 76:862-72 (2008). WB ; Human . Read more (PubMed: 18692027) »

      See all 2 Publications for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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