Overview

  • Product nameAnti-FLASH antibody
    See all FLASH primary antibodies
  • Description
    Rabbit polyclonal to FLASH
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    SERFQQLMKLFEKSKC

    , corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.)

  • Positive control
    • HeLa cell lysate An approximate 220 kDa band can be detected.
  • General notes


    A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesA novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionParticipates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Localizes to discrete nuclear foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA387232 antibody
    • C8AP2_HUMAN antibody
    • CASP 8 associated protein 2 antibody
    • CASP8 associated protein 2 antibody
    • CASP8-associated protein 2 antibody
    • CASP8AP2 antibody
    • Caspase 8 associated protein 2 antibody
    • CED 4 antibody
    • CED4 antibody
    • D4Ertd659e antibody
    • FLASH homolog antibody
    • FLASH homolog RIP 25 antibody
    • FLASH homolog RIP25 antibody
    • FLICE associated huge antibody
    • FLICE associated huge protein antibody
    • FLICE-associated huge protein antibody
    • FLJ11208 antibody
    • KIAA1315 antibody
    • mKIAA1315 antibody
    • OTTMUSP00000005125 antibody
    • RIP 25 antibody
    • RIP25 antibody
    • RP23-69G24.1 antibody
    see all

Anti-FLASH antibody images

  • Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate

    Observed band size : 220 kDa (why is the actual band size different from the predicted?)
  • ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF

References for Anti-FLASH antibody (ab8420)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount 10 µg
Specification LNCaP (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (7% tris-acetate)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2008

Our FLASH antibodies have not yet been tested for IHC and if you wish to test ab8420 for use in immmunocytochemistry, I would be happy to offer you a 30% discount in exchange for IHC information. Please quote reference SMDD1609-30% when placing your or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"