• Product nameAnti-FLCN antibody
    See all FLCN primary antibodies
  • Description
    Rabbit polyclonal to FLCN
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1 - 50 (MNAIVALCHF CELHGPRTLF CTEVLHAPLP QGDGNEDSPG QGEQAEEEEG) of Human FLCN protein (NP_659434.2)

  • Positive control
    • HeLa or 293T whole cell lysates


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7 - 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab87753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionMay play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). May be a tumor suppressor. May be involved in colorectal tumorigenesis. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
  • Tissue specificityExpressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.
  • Involvement in diseaseDefects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD) [MIM:135150]. BHD is a rare autosomal dominant genodermatosis characterized by hair follicle hamartomas (fibrofolliculomas), kidney tumors, and spontaneous pneumothorax. Fibrofolliculomas are part of the triad of BHD skin lesions that also includes trichodiscomas and acrochordons. Onset of this dermatologic condition is invariably in adulthood. BHD is associated with a variety of histologic types of renal tumors, including chromophobe renal cell carcinoma (RCC), benign renal oncocytoma, clear-cell RCC and papillary type I RCC. Multiple lipomas, angiolipomas, and parathyroid adenomas are also seen in patients affected with this disease. The majority of mutations are predicted to prematurely terminate the protein.
    Defects in FLCN are in some cases a cause of primary spontaneous pneumothorax (PSP) [MIM:173600]. PSP is a condition in which air is present in the pleural space in the absence of a precipitating event, such as trauma or lung disease. This results in secondary collapse of the lung, either partially or completely, and some degree of hypoxia. PSP is relatively common, with an incidence between 7.4-18/100'000 for men and 1.2-6/100'000 for women and a dose-dependent, increased risk among smokers. Most cases are sporadic, typically occurring in tall, thin men aged 10-30 years and generally while at rest. Familial PSP is rarer and usually is inherited as an autosomal dominant condition with reduced penetrance, although X-linked recessive and autosomal recessive inheritance have also been suggested.
    Note=Defects in FLCN may be involved in renal cell carcinoma.
  • Sequence similaritiesBelongs to the folliculin family.
  • Developmental stageExpressed in fetal lung, kidney, liver, and brain.
  • Post-translational
    Phosphorylated. Several different phosphorylated forms exist.
  • Cellular localizationCytoplasm. Nucleus. Mainly localized in the nucleus. Co-localizes with FNIP1 and FNIP2 in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BHD antibody
    • BHD skin lesion fibrofolliculoma protein antibody
    • Birt Hogg Dube syndrome protein antibody
    • Birt-Hogg-Dube syndrome protein antibody
    • DKFZp547A118 antibody
    • FLCL antibody
    • Flcn antibody
    • FLCN_HUMAN antibody
    • FLJ45004 antibody
    • FLJ99377 antibody
    • Folliculin antibody
    • MGC17998 antibody
    • MGC23445 antibody
    see all

Anti-FLCN antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FLCN with ab87753 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-FLCN antibody (ab87753) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 64 kDa

    Exposure time : 3 minutes
  • Detection of Human FLCN by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87753 at 3 µg/mg lysate for IP and 1 µg/ml for subsequent WB. Detection: chemiluminescence with an exposure time of 10 seconds.

References for Anti-FLCN antibody (ab87753)

ab87753 has not yet been referenced specifically in any publications.

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