Overview

  • Product nameAnti-FLCN antibody
    See all FLCN primary antibodies
  • Description
    Rabbit polyclonal to FLCN
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of Human FLCN (NP_659434.2).

  • Positive control
    • CEM cell line lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab93196 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 64 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionMay play a role in the pathogenesis of an uncommon form of kidney cancer through its association with an inherited disorder of the hair follicle (fibrofolliculomas). May be a tumor suppressor. May be involved in colorectal tumorigenesis. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
  • Tissue specificityExpressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.
  • Involvement in diseaseDefects in FLCN are the cause of Birt-Hogg-Dube syndrome (BHD) [MIM:135150]. BHD is a rare autosomal dominant genodermatosis characterized by hair follicle hamartomas (fibrofolliculomas), kidney tumors, and spontaneous pneumothorax. Fibrofolliculomas are part of the triad of BHD skin lesions that also includes trichodiscomas and acrochordons. Onset of this dermatologic condition is invariably in adulthood. BHD is associated with a variety of histologic types of renal tumors, including chromophobe renal cell carcinoma (RCC), benign renal oncocytoma, clear-cell RCC and papillary type I RCC. Multiple lipomas, angiolipomas, and parathyroid adenomas are also seen in patients affected with this disease. The majority of mutations are predicted to prematurely terminate the protein.
    Defects in FLCN are in some cases a cause of primary spontaneous pneumothorax (PSP) [MIM:173600]. PSP is a condition in which air is present in the pleural space in the absence of a precipitating event, such as trauma or lung disease. This results in secondary collapse of the lung, either partially or completely, and some degree of hypoxia. PSP is relatively common, with an incidence between 7.4-18/100'000 for men and 1.2-6/100'000 for women and a dose-dependent, increased risk among smokers. Most cases are sporadic, typically occurring in tall, thin men aged 10-30 years and generally while at rest. Familial PSP is rarer and usually is inherited as an autosomal dominant condition with reduced penetrance, although X-linked recessive and autosomal recessive inheritance have also been suggested.
    Note=Defects in FLCN may be involved in renal cell carcinoma.
  • Sequence similaritiesBelongs to the folliculin family.
  • Developmental stageExpressed in fetal lung, kidney, liver, and brain.
  • Post-translational
    modifications
    Phosphorylated. Several different phosphorylated forms exist.
  • Cellular localizationCytoplasm. Nucleus. Mainly localized in the nucleus. Co-localizes with FNIP1 and FNIP2 in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BHD antibody
    • BHD skin lesion fibrofolliculoma protein antibody
    • Birt Hogg Dube syndrome protein antibody
    • Birt-Hogg-Dube syndrome protein antibody
    • DKFZp547A118 antibody
    • FLCL antibody
    • Flcn antibody
    • FLCN_HUMAN antibody
    • FLJ45004 antibody
    • FLJ99377 antibody
    • Folliculin antibody
    • MGC17998 antibody
    • MGC23445 antibody
    see all

Anti-FLCN antibody images

  • Anti-FLCN antibody (ab93196) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 64 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma, staining FLCN with ab93196 at 1/50 dilution. Staining was detected using DAB.

References for Anti-FLCN antibody (ab93196)

ab93196 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (BHD kidney tumor cell lines: UOK257 (FLCN-) & UOK2)
Gel Running Conditions Reduced Denaturing (Bio-Rad 8-16% gradient Tris-Glycine gel)
Loading amount 25 µg
Specification BHD kidney tumor cell lines: UOK257 (FLCN-) & UOK2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted May 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"