Overview

  • Product name
    Anti-FLG2 antibody
  • Description
    Rabbit polyclonal to FLG2
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 280-381 of Human FLG2 (Q5D862).

  • Positive control
    • Human prostate tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the S100-fused protein family.
    In the N-terminal section; belongs to the S-100 family.
    Contains 2 EF-hand domains.
    Contains 10 filaggrin repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • FILA2_HUMAN antibody
    • Filaggrin-2 antibody
    • FLG-2 antibody
    • FLG2 antibody
    • Ifapsoriasin antibody
    • Intermediate filament-associated and psoriasis-susceptibility protein antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

References

ab122011 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
14 µg
Gel Running Conditions
Reduced Denaturing (gel 10%)
Sample
Human Serum (plasma)
Specification
plasma
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Miss. Yi-Tsen Chiang

Verified customer

Submitted Jan 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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