Overview

  • Product nameAnti-FLG2 antibody
  • Description
    Rabbit polyclonal to FLG2
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 280-381 of Human FLG2 (Q5D862).

  • Positive control
    • Human prostate tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Sequence similaritiesBelongs to the S100-fused protein family.
    In the N-terminal section; belongs to the S-100 family.
    Contains 2 EF-hand domains.
    Contains 10 filaggrin repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • FILA2_HUMAN antibody
    • Filaggrin-2 antibody
    • FLG-2 antibody
    • FLG2 antibody
    • Ifapsoriasin antibody
    • Intermediate filament-associated and psoriasis-susceptibility protein antibody
    see all

Anti-FLG2 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

References for Anti-FLG2 antibody (ab122011)

ab122011 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 14 µg
Gel Running Conditions Reduced Denaturing (gel 10%)
Sample Human Serum (plasma)
Specification plasma
Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Miss. Yi-Tsen Chiang

Verified customer

Submitted Jan 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"