Fusion protein, corresponding to the N-terminal and amino acids 1-239 of Mouse FLI1.
Erythroleukemia cell lines (CB7 and DP16-1); human T cell line (Jurkat).
Do not store in a frost-free freezer.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. This concentration is recommended using whole extracts from mouse erythroleukemia cell lines (CB7 and DP16-1) or a human T cell line (Jurkat). Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Involvement in disease
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.