Overview

  • Product name
  • Description
    Sheep polyclonal to FLI1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein, corresponding to the N-terminal and amino acids 1-239 of Mouse FLI1.

  • Positive control
    • Erythroleukemia cell lines (CB7 and DP16-1); human T cell line (Jurkat).
  • General notes
    Do not store in a frost-free freezer. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500. This concentration is recommended using whole extracts from mouse erythroleukemia cell lines (CB7 and DP16-1) or a human T cell line (Jurkat). Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
    • Involvement in disease
      Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
    • Sequence similarities
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERGB transcription factor antibody
      • Ewing Sarcoma breakpoint region 2 antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLI 1 antibody
      • FLI 1 proto oncogene antibody
      • Fli-1 proto-oncogene, ETS transcription factor antibody
      • FLI1 antibody
      • FLI1 EWS fusion gene antibody
      • FLI1 proto oncogene antibody
      • FLI1_HUMAN antibody
      • Friend leukemia integration 1 (FLI1) transcription factor antibody
      • Friend leukemia integration 1 transcription factor antibody
      • Friend leukemia virus integration 1 antibody
      • Proto-oncogene Fli-1 antibody
      • Sarcoma breakpoint region 2 (EWSR2) antibody
      • SIC 1 antibody
      • SIC1 antibody
      • Transcription factor ERGB antibody
      • Viral integration region FLI1 antibody
      • Viral integration region FLI1, mouse, homolog of antibody
      see all

    References

    ab11851 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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