• Product nameAnti-FLI1 antibody
    See all FLI1 primary antibodies
  • Description
    Rabbit polyclonal to FLI1
  • SpecificityThis antibody recognizes FLI1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    A region within synthetic peptide: SGSPDYGQPH KINPLPPQQE WINQPVRVNV KREYDHMNGS RESPVDCSVS, corresponding to N terminal amino acids 37-86 of Human FLI1

  • Positive control
    • Jurkat cell lysate


Associated products


Our Abpromise guarantee covers the use of ab49045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionSequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • Involvement in diseaseDefects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    see all

Anti-FLI1 antibody images

  • Lane 1 : MARKER
    Lane 2 : Anti-FLI1 antibody (ab49045) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-FLI1 antibody (ab49045) at 1/1000 dilution

    Lane 1 : Cytoplasmic lysate prepared from porcine endothelial cells
    Lane 2 : Nuclear lysate prepared from porcine endothelial cells

    Biotin conjugated anti-rabbit at 1/1000 dilution
    developed using the ECL technique

    Predicted band size : 51 kDa

    Exposure time : 1 minute

References for Anti-FLI1 antibody (ab49045)

ab49045 has not yet been referenced specifically in any publications.

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