Overview

  • Product name
    Anti-FLI1 antibody - C-terminal
    See all FLI1 primary antibodies
  • Description
    Rabbit polyclonal to FLI1 - C-terminal
  • Host species
    Rabbit
  • Specificity
    At least four isoforms of FLI1 are known to exist; this antibody will detect all four.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FLI1 (C terminal). The exact sequence is proprietary. 17 amino acid peptide near the C-terminus of Human FLI1.
    Database link: Q01543

  • Positive control
    • Jurkat cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab180902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • Involvement in disease
    Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • Fli-1 proto-oncogene, ETS transcription factor antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 (FLI1) transcription factor antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • Sarcoma breakpoint region 2 (EWSR2) antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    • Viral integration region FLI1, mouse, homolog of antibody
    see all

Images

  • Lane 1 : Anti-FLI1 antibody - C-terminal (ab180902) at 0.5 µg/ml
    Lane 2 : Anti-FLI1 antibody - C-terminal (ab180902) at 1 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 51 kDa
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescent analysis of HeLa cells labeling FLI1 with ab180902 at 5 µg/ml.

References

ab180902 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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