Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
Our Abpromise guarantee covers the use of ab15289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 19289502|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IP||Use a concentration of 2 µg/ml.|
|CHIPseq||Use at an assay dependent concentration. PubMed: 24760698|
|IHC-P||1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
Chromatin was prepared from nuclear lysate of the mouse haematopoietic progenitor cell line HPC-7. The cross-linking (X-ChiP) technique was used, crosslinking was performed for 10 minutes in 0.4% and 1% formaldehyde. The primary antibody was used undiluted and incubated with the sample for 16 hours at 4°C. The immunoprecipitated DNA was quantified by real time PCR.