Overview

  • Product nameAnti-Flightless I antibody [116.40]
    See all Flightless I primary antibodies
  • Description
    Mouse monoclonal [116.40] to Flightless I
  • Specificityab28089 recognises Flightless-I Protein.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab28089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 144 kDa.

Target

  • FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
  • Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
  • Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
  • Sequence similaritiesContains 5 gelsolin-like repeats.
    Contains 15 LRR (leucine-rich) repeats.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fli 1 antibody
    • FLI antibody
    • Fli1 antibody
    • Flightless 1 antibody
    • Flightless I (Drosophila) homolog antibody
    • Flightless I homolog antibody
    • Flightless I homolog (Drosophila) antibody
    • Flightless1 antibody
    • FlightlessI antibody
    • fliI antibody
    • FLII_HUMAN antibody
    • Fliih antibody
    • FLIL antibody
    • MGC39265 antibody
    • Protein flightless 1 homolog antibody
    • Protein flightless-1 homolog antibody
    see all

References for Anti-Flightless I antibody [116.40] (ab28089)

This product has been referenced in:
  • Ambrosino C  et al. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Mol Cell Proteomics 9:1352-67 (2010). Read more (PubMed: 20308691) »
  • Goodall AH  et al. Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function. Blood 116:4646-4656 (2010). WB ; Human . Read more (PubMed: 20833976) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse monocytes)
Loading amount 20 µg
Specification Mouse monocytes
Treatment LPS for 24hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Dec 18 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"