The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 144 kDa.
FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
References for Anti-Flightless I antibody [116.40] (ab28089)
This product has been referenced in:
Ambrosino C et al. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Mol Cell Proteomics9:1352-67 (2010).
Read more (PubMed: 20308691) »
Goodall AH et al. Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function. Blood116:4646-4656 (2010).
Read more (PubMed: 20833976) »