Overview

  • Product nameAnti-Flightless I antibody
    See all Flightless I primary antibodies
  • Description
    Rabbit polyclonal to Flightless I
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 425 - 475 of Human Flightless I (NP_002009.1)

  • Positive control
    • Breast carcinoma, colon carcinoma, ovarian carcinoma, prostate carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
    • Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
    • Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
    • Sequence similaritiesContains 5 gelsolin-like repeats.
      Contains 15 LRR (leucine-rich) repeats.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Fli 1 antibody
      • FLI antibody
      • Fli1 antibody
      • Flightless 1 antibody
      • Flightless I (Drosophila) homolog antibody
      • Flightless I homolog antibody
      • Flightless I homolog (Drosophila) antibody
      • Flightless1 antibody
      • FlightlessI antibody
      • fliI antibody
      • FLII_HUMAN antibody
      • Fliih antibody
      • FLIL antibody
      • MGC39265 antibody
      • Protein flightless 1 homolog antibody
      • Protein flightless-1 homolog antibody
      see all

    Anti-Flightless I antibody images

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma, using 1/100 ab84778 in conjunction with 1/100 Goat anti-Rabbit IgG secondary antibody conjugated to a fluorescent dye (red).
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma, using 1/250 ab84778 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma, using 1/250 ab84778 followed by DAB staining.

    References for Anti-Flightless I antibody (ab84778)

    ab84778 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84778.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"