Overview

  • Product nameAnti-Flightless I antibody
    See all Flightless I primary antibodies
  • Description
    Rabbit polyclonal to Flightless I
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 435-748 of Human Flightless I (NP_002009).

  • Positive control
    • WB: 293T, H1299, HeLaS3, MOLT4 and Raji cell lysates ICC/If: Hela cell

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 145 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
  • Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
  • Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
  • Sequence similaritiesContains 5 gelsolin-like repeats.
    Contains 15 LRR (leucine-rich) repeats.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fli 1 antibody
    • FLI antibody
    • Fli1 antibody
    • Flightless 1 antibody
    • Flightless I (Drosophila) homolog antibody
    • Flightless I homolog antibody
    • Flightless I homolog (Drosophila) antibody
    • Flightless1 antibody
    • FlightlessI antibody
    • fliI antibody
    • FLII_HUMAN antibody
    • Fliih antibody
    • FLIL antibody
    • MGC39265 antibody
    • Protein flightless 1 homolog antibody
    • Protein flightless-1 homolog antibody
    see all

Anti-Flightless I antibody images

  • Anti-Flightless I antibody (ab96314) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 145 kDa
  • ab96314 at 1/200 dilution staining Flightless I in paraformaldehyde fixed HeLa cells by Immunofluorescence.
    The lower image shows ab96314 merged with a DNA probe.

References for Anti-Flightless I antibody (ab96314)

ab96314 has not yet been referenced specifically in any publications.

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