Recombinant Anti-Flightless I antibody [EPR4201] (ab108594)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4201] to Flightless I
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Flightless I antibody [EPR4201]
See all Flightless I primary antibodies -
Description
Rabbit monoclonal [EPR4201] to Flightless I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A549 and A431 cell lysates. IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4201 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 145 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ICC/IF
1/100 - 1/250. |
Target
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Function
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration. -
Tissue specificity
Strongest expression in skeletal muscle with high expression also in the heart and lung. -
Involvement in disease
Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems. -
Sequence similarities
Contains 5 gelsolin-like repeats.
Contains 15 LRR (leucine-rich) repeats. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes. - Information by UniProt
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Database links
- Entrez Gene: 2314 Human
- Entrez Gene: 14248 Mouse
- Entrez Gene: 287375 Rat
- Omim: 600362 Human
- SwissProt: Q13045 Human
- SwissProt: Q9JJ28 Mouse
- Unigene: 513984 Human
- Unigene: 339755 Mouse
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Alternative names
- Fli 1 antibody
- FLI antibody
- Fli1 antibody
see all
Images
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All lanes : Anti-Flightless I antibody [EPR4201] (ab108594) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa -
ab108594 at 1/100 dilution staining Flightless I in HeLa cells by Immunofluorescence.
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ab108594 at 1/100 dilution staining Flightless I in Human skeletal muscle by Immunohistochemistry, Paraffin-embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108594 has been referenced in 2 publications.
- Alarcón-Sánchez BR et al. Nucleoredoxin interaction with flightless-I/actin complex is differentially altered in alcoholic liver disease. Basic Clin Pharmacol Toxicol 127:389-404 (2020). PubMed: 32524749
- Arora PD et al. Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling. Mol Biol Cell 26:2279-97 (2015). IP . PubMed: 25877872