Anti-Flightless I antibody [EPR4202(2)] (ab109015)

Overview

  • Product nameAnti-Flightless I antibody [EPR4202(2)]
    See all Flightless I primary antibodies
  • Description
    Rabbit monoclonal [EPR4202(2)] to Flightless I
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Human heart, HeLa, A431, and A549 lysates; Human muscle tissue; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMay play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration.
    • Tissue specificityStrongest expression in skeletal muscle with high expression also in the heart and lung.
    • Involvement in diseaseDeletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems.
    • Sequence similaritiesContains 5 gelsolin-like repeats.
      Contains 15 LRR (leucine-rich) repeats.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Fli 1 antibody
      • FLI antibody
      • Fli1 antibody
      • Flightless 1 antibody
      • Flightless I (Drosophila) homolog antibody
      • Flightless I homolog antibody
      • Flightless I homolog (Drosophila) antibody
      • Flightless1 antibody
      • FlightlessI antibody
      • fliI antibody
      • FLII_HUMAN antibody
      • Fliih antibody
      • FLIL antibody
      • MGC39265 antibody
      • Protein flightless 1 homolog antibody
      • Protein flightless-1 homolog antibody
      see all

    Anti-Flightless I antibody [EPR4202(2)] images

    • All lanes : Anti-Flightless I antibody [EPR4202(2)] (ab109015) at 1/1000 dilution

      Lane 1 : Humam heart lysate
      Lane 2 : HeLa lysate
      Lane 3 : A431 lysate
      Lane 4 : A549 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 145 kDa
    • Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.
    • Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution.

    References for Anti-Flightless I antibody [EPR4202(2)] (ab109015)

    ab109015 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"