The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human FLIP peptide (ab8457).
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains.
The caspase domain lacks the active sites residues involved in catalysis.
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
CASP8 and FADD-like apoptosis regulator subunit p12 antibody
Caspase homolog antibody
Caspase like apoptosis regulatory protein antibody
Caspase related inducer of apoptosis antibody
Caspase-eight-related protein antibody
Caspase-like apoptosis regulatory protein antibody
Cellular FLICE-like inhibitory protein antibody
FADD like anti apoptotic molecule antibody
FADD-like antiapoptotic molecule 1 antibody
I FLICE antibody
Inhibitor of FLICE antibody
MACH-related inducer of toxicity antibody
Usurpin beta antibody
Western blot - FLIP antibody (ab84283)
All lanes : Anti-FLIP antibody (ab84283) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa Observed band size: 55 kDa Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Immunocytochemistry/ Immunofluorescence - FLIP antibody (ab84283)Image part of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada
ab84283 (1/200) staining FLIP in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised using 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.