Overview

  • Product nameAnti-FLIP delta + gamma antibody
  • Description
    Rabbit polyclonal to FLIP delta + gamma
  • SpecificityThis antibody recognizes human and murine FLIP gamma and FLIP delta It does not recognise FLIP alpha and FLIP beta.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    QKSLKDPSNNFRMITPYAH

    , corresponding to C terminal amino acids 191/209 of Human FLIP delta + gamma. (Peptide available as ab8458).

  • Positive control
    • Jurkat cell lysate. 35 kDa and 25 kDa bands can bedetected.
  • General notes


    Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively (1,2). The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIP alpha, beta, gamma, and delta, respectively (8).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively (1,2). The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIP alpha, beta, gamma, and delta, respectively (8).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 35 kDa.Can be blocked with Human FLIP peptide (ab8458).
ICC/IF Use a concentration of 2 µg/ml.

Target

  • RelevanceFLIP (or CASP8 and FADD-like apoptosis regulator) is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CASP8 and FADD like apoptosis regulator antibody
    • Caspase like apoptosis regulatory protein antibody
    • Cellular FLICE like inhibitory protein antibody
    • CFLAR antibody
    • FLAME 1 antibody
    • FLAME 1 delta antibody
    • FLICE antibody
    • Usurpin gamma antibody
    see all

Anti-FLIP delta + gamma antibody images

  • All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/1000 dilution

    Lane 1 : HeLa total cell lysate
    Lane 2 : Jurkat total cell lysate
    Lane 3 : THP1 total cell lysate
    Lane 4 : A431 total cell lysate
    Lane 5 : K562 total cell lysate
    Lane 6 : NIH3T3 cells


    Observed band size : 25,35 kDa (why is the actual band size different from the predicted?)
  • ab8423 at 2µg/ml staining FLIP delta + gamma in Hela cells by ICC/IF

  • All lanes : Anti-FLIP delta + gamma antibody (ab8423) at 1/5000 dilution

    Lane 1 : TF-1 whole cell lysate
    Lane 2 : TF-1 whole cell lysate
    Lane 3 : TF-1 whole cell lysate
    Lane 4 : Jurkat whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 30,44,98 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-FLIP delta + gamma antibody (ab8423)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (TF-1 cells)
Loading amount 30 µg
Specification TF-1 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5%
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Submitted Jul 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"