Overview

  • Product nameAnti-FLJ20259 antibody
  • Description
    Rabbit polyclonal to FLJ20259
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen: EEHIPHQQIQ AQLVAGQSLA GGQQIQIQTV GALSPPPSQQ GSPREGERRV GTASVLQPVK KRKVDMPITV SYAISGQPVA TVLAIPQGQ, corresponding to amino acids 187-275 of Human FLJ20259 according to Q2TAL8.

  • Positive control
    • Human pancreas tissue; Human liver, Human tonsil, RT-4 and U-251 MG cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-FLJ20259 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122405 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122405, at a 1/150 dilution, staining FLJ20259 in glandular cells of paraffin-embedded Human pancreas tissue by immunohistochemistry.
  • All lanes : Anti-FLJ20259 antibody (ab122405) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-FLJ20259 antibody (ab122405)

ab122405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"