Overview

  • Product nameAnti-FLJ20530 antibody
    See all FLJ20530 primary antibodies
  • Description
    Rabbit polyclonal to FLJ20530
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    Synthetic peptide (Human) - the immunogen lies in the region between residues 200 and 250 of human FLJ20530/ KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656).

Properties

Applications

Our Abpromise guarantee covers the use of ab18050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 120 kDa.

Target

  • RelevanceFLJ20530 is a component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3' box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • C8orf52 antibody
    • Chromosome 8 open reading frame 52 antibody
    • FLJ20530 antibody
    • INT8 antibody
    • Integrator complex subunit 8 antibody
    • INTS 8 antibody
    • INTS8 antibody
    • KAONASHI protein 1 antibody
    • KAONASHI1 antibody
    • MGC131633 antibody
    see all

Anti-FLJ20530 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FLJ20530 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot using ab18050 at 0.2µg/ml on HeLa nuclear lysate.

    Lysate: 5µg/lane.
    Detection: Chemiluminescence with an exposure time of 20 minutes. Western blot using ab18050 at 0.2µg/ml on HeLa nuclear lysate.

    Lysate: 5µg/lane.
    Detection: Chemiluminescence with an exposure time of 20 minutes.

References for Anti-FLJ20530 antibody (ab18050)

ab18050 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"