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Synthetic peptide (Human) - the immunogen lies in the region between residues 200 and 250 of human FLJ20530/ KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656).
Our Abpromise guarantee covers the use of ab18050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 120 kDa.|
Detection: Chemiluminescence with an exposure time of 20 minutes.
ab18050 has not yet been referenced specifically in any publications.