Overview

  • Product nameAnti-FLJ22167 antibody
    See all FLJ22167 primary antibodies
  • Description
    Rabbit polyclonal to FLJ22167
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: FNSGFWLKRS SYEEQPTVRF QHQVLLVALL GPESDGFLAW STFPAFNRLQ, corresponding to amino acids 73-122 of Human FLJ22167

  • Positive control
    • Human kidney tissue and HepG2 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab62475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionTransmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling.
  • Involvement in diseaseDefects in TMEM231 are the cause of Joubert syndrome 20 (JBTS20) [MIM:614970]. A disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease.
  • Sequence similaritiesBelongs to the TMEM231 family.
  • Cellular localizationCell projection > cilium membrane. Localizes to the transition zone of primary cilia; SEPT2 is required for localization to the transition zone.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALYE870 antibody
    • CDNA: FLJ22167 fis, clone HRC00584 antibody
    • hCG 2043031 antibody
    • HCG2043031, isoform CRA a antibody
    • hypothetical protein LOC79583 antibody
    • JBTS20 antibody
    • PRO1886 antibody
    • Putative uncharacterized protein FLJ22167 antibody
    • Q9H6L2 antibody
    • TM231_HUMAN antibody
    • Tmem231 antibody
    • Transmembrane protein 231 antibody
    • UNQ870/PRO1886 antibody
    • UPF0513 membrane protein antibody
    see all

Anti-FLJ22167 antibody images

  • All lanes : Anti-FLJ22167 antibody (ab62475) at 1.25 µg/ml

    Lane 1 : marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FLJ22167 with ab62475 at 4-8µg/ml. Arrows indicate positively labelled renal tubule epithelial cells. Magnification: 400X.

References for Anti-FLJ22167 antibody (ab62475)

ab62475 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62475.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"