The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPhospholipase acting on various phospholipids including phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and lysophospholipids. May have a role in the defense against invading microorganisms and in the generation of lipid mediators of inflammation.
Tissue specificityExpressed in neutrophils and monocytes.
Sequence similaritiesBelongs to the phospholipase B-like family.
Post-translational modificationsProteolytic processing leading to a 22 kDa N-terminal and a 42 kDa C-terminal fragment appears necessary for activity, which seems to derive from the 42 kDa chain.