Anti-FLJ35220 antibody (ab69400)
Key features and details
- Mouse polyclonal to FLJ35220
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FLJ35220 antibody -
Description
Mouse polyclonal to FLJ35220 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human FLJ35220 aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- FLJ35220 transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Relevance
This gene encodes hypothetical protein FLJ35220 -
Database links
- Entrez Gene: 284131 Human
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Alternative names
- hypothetical protein FLJ35220 antibody
- hypothetical protein LOC284131 antibody
- MGC74455 antibody
Images
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All lanes : Anti-FLJ35220 antibody (ab69400) at 1/500 dilution
Lane 1 : FLJ35220 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
ab69400 at 10µg/ml staining FLJ35220 in Hela cells by ICC/IF.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab69400 has been referenced in 3 publications.
- Kong XY et al. Endonuclease V Regulates Atherosclerosis Through C-C Motif Chemokine Ligand 2-Mediated Monocyte Infiltration. J Am Heart Assoc 10:e020656 (2021). PubMed: 34259011
- Berges N et al. Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform. PLoS One 14:e0225081 (2019). PubMed: 31703097
- Nawaz MS et al. Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules. J Biol Chem 291:21786-21801 (2016). PubMed: 27573237