Overview

  • Product name
    Anti-FLJ35220 antibody
  • Description
    Mouse polyclonal to FLJ35220
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-264 of Human FLJ35220 (AAH64545.1)

  • Positive control
    • FLJ35220 transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Relevance
    This gene encodes hypothetical protein FLJ35220
  • Database links
  • Alternative names
    • hypothetical protein FLJ35220 antibody
    • hypothetical protein LOC284131 antibody
    • MGC74455 antibody

Images

  • All lanes : Anti-FLJ35220 antibody (ab69400) at 1/500 dilution

    Lane 1 : FLJ35220 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ab69400 at 10µg/ml staining FLJ35220 in Hela cells by ICC/IF.

References

This product has been referenced in:
  • Nawaz MS  et al. Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules. J Biol Chem 291:21786-21801 (2016). Read more (PubMed: 27573237) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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