Overview

  • Product name
  • Description
    Rabbit polyclonal to FLJ36733
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: FKFICDASSL HQVRKKLDSL GLCSVSCALE FIPNSKVQLA EPDLEQAAHL IQALSNHEDV IHVYDNI, corresponding to C terminal amino acids 230-296 of Human FLJ36733.

  • Positive control
    • Human kidney tissue; U 2 OS cells; RT 4 and U 251 MG lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab121688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • Function
    Acts as a translational activator of mitochondrially-encoded cytochrome c oxidase 1.
  • Involvement in disease
    Defects in TACO1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency.
  • Sequence similarities
    Belongs to the TACO1 family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCDC44 antibody
    • CDNA FLJ36733 fis antibody
    • Coiled coil domain containing 44 antibody
    • Coiled-coil domain-containing protein 44 antibody
    • PRO0477 antibody
    • Taco1 antibody
    • TACO1_HUMAN antibody
    • Translational activator of cytochrome c oxidase 1 antibody
    • Translational activator of mitochondrially-encoded cytochrome c oxidase I antibody
    • UTERU2012856 antibody
    see all

Images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-FLJ36733 antibody (ab121688) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab121688 at 1/75 dilution staining FLJ36733 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab121688 at 4 µg/ml staining FLJ36733 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References

This product has been referenced in:
  • Lee DE  et al. Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice. Acta Physiol (Oxf) 218:167-177 (2016). Read more (PubMed: 27061106) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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