Detection of Flotillin 1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab123512 at 6µg/mg lysate for IP (Lane 1). For WB detection an anti-Flotillin 1 antibody which recognizes an downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-Flotillin 1 antibody (ab123512)
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