Overview

  • Product nameAnti-Flotillin 1 antibody
    See all Flotillin 1 primary antibodies
  • Description
    Rabbit polyclonal to Flotillin 1
  • Tested applicationsSuitable for: IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 150-200 of Human Flotillin 1 (NP_005794.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab123512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

Target

Anti-Flotillin 1 antibody images

  • Detection of Flotillin 1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab123512 at 6µg/mg lysate for IP (Lane 1).
    For WB detection an anti-Flotillin 1 antibody which recognizes an downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-Flotillin 1 antibody (ab123512)

ab123512 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"