Overview

  • Product nameAnti-Flotillin 1 antibody
    See all Flotillin 1 primary antibodies
  • Description
    Rabbit polyclonal to Flotillin 1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Macaque Monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in Rat and differs by one amino acid in Human

  • Positive control
    • Human HEK-293T cells and whole cell extract of mouse/rat lung.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 - 10 µg/ml (recommended with Human HEK-293T cells).
    WB: Use at a concentration of 0.5 - 2.0 µg/ml (recommended with whole cell extract of mouse/rat lung). Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Flotillin 1 antibody images

    • Indirect Immunofluorescence. Human 293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 10 µg/mL Anti-Flotillin 1. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.

    References for Anti-Flotillin 1 antibody (ab50671)

    This product has been referenced in:
    • Jerman S  et al. OFD1 and flotillins are integral components of a ciliary signaling protein complex organized by polycystins in renal epithelia and odontoblasts. PLoS One 9:e106330 (2014). Mouse . Read more (PubMed: 25180832) »
    • Monje FJ  et al. Flotillin-1 is an evolutionary-conserved memory-related protein up-regulated in implicit and explicit learning paradigms. Ann Med 45:301-7 (2013). WB ; Mouse . Read more (PubMed: 23631399) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab50671.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"