Mouse, Rat, Human Predicted to work with:
Cow, Pig, Macaque Monkey, Rhesus monkey, Orangutan
Synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in Rat and differs by one amino acid in Human
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 5 - 10 µg/ml (recommended with Human HEK-293T cells).
WB: Use at a concentration of 0.5 - 2.0 µg/ml (recommended with whole cell extract of mouse/rat lung). Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Sequence similaritiesBelongs to the band 7/mec-2 family. Flotillin subfamily.
Cellular localizationCell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Indirect Immunofluorescence. Human 293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 10 µg/mL Anti-Flotillin 1. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
References for Anti-Flotillin 1 antibody (ab50671)
This product has been referenced in:
Jerman S et al. OFD1 and flotillins are integral components of a ciliary signaling protein complex organized by polycystins in renal epithelia and odontoblasts. PLoS One9:e106330 (2014).
Read more (PubMed: 25180832) »
Monje FJ et al. Flotillin-1 is an evolutionary-conserved memory-related protein up-regulated in implicit and explicit learning paradigms. Ann Med45:301-7 (2013).
Read more (PubMed: 23631399) »