Anti-Flotillin 1 antibody - C-terminal (ab228585)
Key features and details
- Rabbit polyclonal to Flotillin 1 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
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Overview
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Product name
Anti-Flotillin 1 antibody - C-terminal
See all Flotillin 1 primary antibodies -
Description
Rabbit polyclonal to Flotillin 1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig, Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide within Human Flotillin 1 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: O75955 -
Positive control
- WB: HepG2 whole cell lysate. IP: HCT-116 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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Notes |
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IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
Target
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Function
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. -
Sequence similarities
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
Cellular localization
Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 10211 Human
- Omim: 606998 Human
- SwissProt: O75955 Human
- SwissProt: Q767L6 Pig
- Unigene: 179986 Human
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Alternative names
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
see all
Images
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Anti-Flotillin 1 antibody - C-terminal (ab228585) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 47 kDa10% SDS-PAGE
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Flotillin 1 was immunoprecipitated from HCT-116 (human colorectal carcinoma cell line) whole cell extract with 4 μg ab228585. Western blot was performed from the immunoprecipitate using ab228585 at 1/1000 dilution.
Lane 1: Control pre-immune IgG IP in HCT-116 whole cell extract.
Lane 2: ab228585 IP in HCT-116 whole cell extract.
10% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228585 has not yet been referenced specifically in any publications.