Anti-Flotillin 1 antibody [EP446Y] (ab78178)

Overview

  • Product nameAnti-Flotillin 1 antibody [EP446Y]
    See all Flotillin 1 primary antibodies
  • Description
    Rabbit monoclonal [EP446Y] to Flotillin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Flotillin 1 aa 50-150.
    Database link: O75955

  • Positive control
    • K562 cell lysate. C2C12 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab78178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 48 kDa.

For unpurified, use 1/500 - 1/1000.

Target

  • FunctionMay act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • Sequence similaritiesBelongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localizationCell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLOT 1 antibody
    • FLOT1 antibody
    • FLOT1_HUMAN antibody
    • Flotillin-1 antibody
    • Flotillin1 antibody
    • Integral membrane component of caveolae antibody
    • Reggie 2 antibody
    see all

Anti-Flotillin 1 antibody [EP446Y] images

  • All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

    Lane 1 : mouse brain lysate at 10 µg
    Lane 2 : rat brain lysate at 20 µg
    Lane 3 : C6 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution (unpurified) + K562 cell lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References for Anti-Flotillin 1 antibody [EP446Y] (ab78178)

ab78178 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab78178.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"