Overview

  • Product name
  • Description
    Rabbit polyclonal to Flotillin 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    IKKAT

    , corresponding to a region around amino acids 420-424 of Human Esa1 (Q14254).

  • Positive control
    • JK, HUVEC, HepG2 and HeLa cell extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab123376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking over night.

Target

  • Function
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • Tissue specificity
    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • Sequence similarities
    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • Cellular localization
    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen 1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • ESA1 antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin 2 (epidermal surface antigen 1) antibody
    • Flotillin-2 antibody
    • M17S1 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • Membrane component chromosome 17 surface marker 1 homolog antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
    • Membrane component, chromosome 17, surface marker 1 antibody
    • REG-1 antibody
    • Reggie-1 antibody
    • Reggie-2 antibody
    see all

Images

  • All lanes : Anti-Flotillin 2 antibody (ab123376) at 1/500 dilution

    Lane 1 : JK cell extract
    Lane 2 : HUVEC cell extract
    Lane 3 : HepG2 cell extract
    Lane 4 : HeLa cell extract


    Predicted band size : 47 kDa

References

ab123376 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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