• Product nameAnti-Flt3 / CD135 antibody
    See all Flt3 / CD135 primary antibodies
  • Description
    Rabbit polyclonal to Flt3 / CD135
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Flt3/ CD135.

    (Peptide available as ab68602.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: SW480, MDA-MB-361, HeLa and MCF7. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.



Our Abpromise guarantee covers the use of ab66035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 113 kDa).


  • FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
  • Tissue specificityBone marrow cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 135 antibody
    • CD135 antibody
    • CD135 antigen antibody
    • Fetal liver kinase 2 antibody
    • FL cytokine receptor antibody
    • Flk 2 antibody
    • Flk2 antibody
    • Flt 3 antibody
    • FLT-3 antibody
    • Flt3 antibody
    • FLT3_HUMAN antibody
    • FMS like tyrosine kinase 3 antibody
    • Fms related tyrosine kinase 3 antibody
    • Fms-like tyrosine kinase 3 antibody
    • Growth factor receptor tyrosine kinase type III antibody
    • Ly-72 antibody
    • OTTHUMP0000004234 antibody
    • Receptor type tyrosine protein kinase FLT3 antibody
    • Stem cell tyrosine kinase 1 antibody
    • Stk 1 antibody
    • STK-1 antibody
    • Stk1 antibody
    • Tyrosine protein kinase receptor FLT3 antibody
    • Tyrosine-protein kinase receptor FLT3 antibody
    see all

Anti-Flt3 / CD135 antibody images

  • All lanes : Anti-Flt3 / CD135 antibody (ab66035) at 1 µg/ml

    Lane 1 : SW480 whole cell lysate (ab3957)
    Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 113 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,36 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of Flt3 / CD135 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66035, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Flt3 / CD135 antibody (ab66035)

ab66035 has not yet been referenced specifically in any publications.

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