Anti-Flt3 / CD135 antibody - Aminoterminal end (ab37847)

Overview

  • Product nameAnti-Flt3 / CD135 antibody - Aminoterminal end
    See all Flt3 / CD135 primary antibodies
  • Description
    Rabbit polyclonal to Flt3 / CD135 - Aminoterminal end
  • Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from aa 48-65 (

    GKSSSYPMVSESPEDLGC

    ) of human Flt3/ CD135.

  • Positive control
    • HL-60 cell lysate. breast carcinoma This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab37847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt 1/1 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for the FL cytokine. Has a tyrosine-protein kinase activity.
  • Tissue specificityBone marrow cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 135 antibody
    • CD135 antibody
    • CD135 antigen antibody
    • Fetal liver kinase 2 antibody
    • FL cytokine receptor antibody
    • Flk 2 antibody
    • Flk2 antibody
    • Flt 3 antibody
    • FLT-3 antibody
    • Flt3 antibody
    • FLT3_HUMAN antibody
    • FMS like tyrosine kinase 3 antibody
    • Fms related tyrosine kinase 3 antibody
    • Fms-like tyrosine kinase 3 antibody
    • Growth factor receptor tyrosine kinase type III antibody
    • Ly-72 antibody
    • OTTHUMP0000004234 antibody
    • Receptor type tyrosine protein kinase FLT3 antibody
    • Stem cell tyrosine kinase 1 antibody
    • Stk 1 antibody
    • STK-1 antibody
    • Stk1 antibody
    • Tyrosine protein kinase receptor FLT3 antibody
    • Tyrosine-protein kinase receptor FLT3 antibody
    see all

Anti-Flt3 / CD135 antibody - Aminoterminal end images

  • Flow cytometric analysis of 293 cells, staining Flt3 / CD135 (green) with ab37847 at 1/10 dilution. A FITC-conjugated goat-anti-rabbit secondary was used.

  • Flow Cytometry analysis of Flt3 / CD135 in human endothelial precursor cells using ab37847 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.
    Red line= ab37847
    Black line= isotype control

    See Abreview

  • ab37847 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37847 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Flt3 / CD135 antibody - Aminoterminal end (ab37847)

ab37847 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (endothelial precursor cell)
Specification endothelial precursor cell
Fixation none
Permeabilization No
Gating Strategy CD133 and CD202B
Username

Dr. A Jackson

Verified customer

Submitted Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"