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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Protein G purified
Purification notes This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
1/50 - 1/100.
Function Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.
Tissue specificity Bone marrow cells.
Sequence similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 protein kinase domain.
Cellular localization Membrane.
Information by UniProt
CD 135 antibody
CD135 antigen antibody
Anti-Flt3 / CD135 antibody - Carboxyterminal end images
Western blot - Flt3 / CD135 antibody (ab71771)
Anti-Flt3 / CD135 antibody - Carboxyterminal end (ab71771) at 1/100 dilution + 293 cell lysate at 12.5 µg Secondary Anti rabbit HRP developed using the ECL technique Predicted band size : 113 kDa Observed band size : 113 kDa Additional bands at : 35 kDa,49 kDa,74 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Flt3 / CD135 antibody (ab71771)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab71771 at 1/50 dilution followed by use of a peroxidase-conjugated secondary antibody and AEC staining.
References for Anti-Flt3 / CD135 antibody - Carboxyterminal end (ab71771)
has not yet been referenced specifically in any publications.
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