• Product nameAnti-Flt3 ligand antibody [EP1140Y]
    See all Flt3 ligand primary antibodies
  • Description
    Rabbit monoclonal [EP1140Y] to Flt3 ligand
  • SpecificityThe antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    A synthetic peptide corresponding to residues within human Flt3 ligand.

  • Positive control
    • IHC: Human tonsil tissue. WB: recombinant protein
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/100000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionStimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FL antibody
      • Flt 3 ligand antibody
      • Flt 3L antibody
      • Flt3 L antibody
      • FLT3 LG antibody
      • Flt3 ligand antibody
      • Flt3L antibody
      • FLT3L_HUMAN antibody
      • Flt3lg antibody
      • Fms related tyrosine kinase 3 ligand antibody
      • Fms-related tyrosine kinase 3 ligand antibody
      • SL cytokine antibody
      see all

    Anti-Flt3 ligand antibody [EP1140Y] images

    • ab52648 staining Flt3 ligand in Rhesus monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in 20% Normanl Goat serum in 1x TTBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Anti-Flt3 ligand antibody [EP1140Y] (ab52648) at 1/100000 dilution + Recombinant Flr3 ligand at 10 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 26 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.

    References for Anti-Flt3 ligand antibody [EP1140Y] (ab52648)

    This product has been referenced in:
    • Taylor RM  et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). Read more (PubMed: 22906984) »
    • Tobón GJ  et al. The Fms-like tyrosine kinase 3 ligand, a mediator of B cell survival, is also a marker of lymphoma in primary Sjögren's syndrome. Arthritis Rheum 62:3447-56 (2010). IF ; Human . Read more (PubMed: 20556820) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
    Sample Human Tissue sections (liver tumor)
    Specification liver tumor
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 08 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rhesus monkey Tissue sections (Brain)
    Specification Brain
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Mar 28 2013