Anti-Fluorescein antibody [6A4] (ab6213)
Key features and details
- Mouse monoclonal [6A4] to Fluorescein
- Suitable for: WB, ELISA, ICC
- Reacts with: Species independent
- Isotype: IgM
Overview
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Product name
Anti-Fluorescein antibody [6A4]
See all Fluorescein primary antibodies -
Description
Mouse monoclonal [6A4] to Fluorescein -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Fluorescein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
6A4 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000.
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ELISA |
1/2000 - 1/20000.
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|
ICC |
1/200 - 1/2000.
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Notes |
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WB
1/500 - 1/5000. |
ELISA
1/2000 - 1/20000. |
ICC
1/200 - 1/2000. |
Target
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Relevance
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
Alternative names
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab6213 has been referenced in 2 publications.
- Maheswaranathan M et al. Platelet-derived Growth Factor (PDGF) Regulates Slingshot Phosphatase Activity via Nox1-dependent Auto-dephosphorylation of Serine 834 in Vascular Smooth Muscle Cells. J Biol Chem 286:35430-7 (2011). IP . PubMed: 21857021
- Odagi I et al. Examination of normal intestine using confocal endomicroscopy. J Gastroenterol Hepatol 22:658-62 (2007). PubMed: 17444852