Overview

  • Product nameAnti-Fluorescein antibody [6A4]
    See all Fluorescein primary antibodies
  • Description
    Mouse monoclonal [6A4] to Fluorescein
  • Tested applicationsSuitable for: WB, ELISA, IP, ICCmore details
  • Immunogen

    Synthetic peptide corresponding to Fluorescein.

  • General notesHuman synaptosome associated protein 23 (SNAP23) MW is 27 kDa

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number6A4
  • Myelomaunknown
  • IsotypeIgM
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/2000 - 1/20000.
IP Use at an assay dependent concentration. PubMed: 21857021
ICC 1/200 - 1/2000.

Target

  • RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References for Anti-Fluorescein antibody [6A4] (ab6213)

This product has been referenced in:
  • Maheswaranathan M  et al. Platelet-derived Growth Factor (PDGF) Regulates Slingshot Phosphatase Activity via Nox1-dependent Auto-dephosphorylation of Serine 834 in Vascular Smooth Muscle Cells. J Biol Chem 286:35430-7 (2011). IP . Read more (PubMed: 21857021) »
  • Odagi I  et al. Examination of normal intestine using confocal endomicroscopy. J Gastroenterol Hepatol 22:658-62 (2007). Read more (PubMed: 17444852) »

See all 2 Publications for this product

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I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in...

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Thank you for your enquiry. I am sorry that the answer has taken so long. The answer from the source of the antiserum has not been forthcoming. Unfortunately I have been informed that ab6213's cross reactivity with carboxyfluorescein has never been...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"