Anti-Fluorescein antibody (Alkaline Phosphatase) (ab6657)

Overview

  • Product nameAnti-Fluorescein antibody (Alkaline Phosphatase)
    See all Fluorescein primary antibodies
  • Description
    Goat polyclonal to Fluorescein (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Fluorescein conjugated to Goat IgG

  • General notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8
    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000 - 1/5000.
ELISA 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
WB 1/1000 - 1/5000.

Target

  • RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References for Anti-Fluorescein antibody (Alkaline Phosphatase) (ab6657)

ab6657 has not yet been referenced specifically in any publications.

Product Wall

Based on the Lot #6189 of ab6657, a working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product (assayed against 1.0 ug of Fluorescein conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"