Overview

  • Product name
    Anti-Fluorescein antibody (Biotin)
    See all Fluorescein primary antibodies
  • Description
    Goat polyclonal to Fluorescein (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details
  • Immunogen

    Fluorescein conjugated to Goat IgG

  • General notes
    For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent concentration.
In situ hybridization Use at an assay dependent concentration.
WB 1/2000 - 1/10000.

Target

  • Relevance
    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (Biotin) images

  • Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.

References for Anti-Fluorescein antibody (Biotin) (ab6655)

This product has been referenced in:
  • Long K  et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl . Read more (PubMed: 26838601) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

The antibodies are from different animals and are manufactured in different laboratories so we can expect some variation. The product ab19224 is against FITC which is a derivative of Flouroscein.

I hope th...

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Thank you for your enquiry. I'm sorry but we don't have any information about this antibody being able to detect Fluorescein Sodium. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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