Biotin Anti-Fluorescein antibody [BT.6A4] (ab6215)
Key features and details
- Biotin Mouse monoclonal [BT.6A4] to Fluorescein
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgM
Overview
-
Product name
Biotin Anti-Fluorescein antibody [BT.6A4]
See all Fluorescein primary antibodies -
Description
Biotin Mouse monoclonal [BT.6A4] to Fluorescein -
Host species
Mouse -
Conjugation
Biotin -
Specificity
The antibody reacts with free and bound fluorescein. -
Tested applications
Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details -
Species reactivity
Reacts with: Species independent -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
-
Purification notes
Purified anti-fluorescein biotin conjugate. -
Clonality
Monoclonal -
Clone number
BT.6A4 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/5000.
|
|
ELISA |
1/1000 - 1/10000.
|
|
IHC-P |
1/200 - 1/2000.
|
|
IHC-Fr |
1/200 - 1/2000.
|
|
In situ hybridization |
Use at an assay dependent dilution.
|
Notes |
---|
WB
1/500 - 1/5000. |
ELISA
1/1000 - 1/10000. |
IHC-P
1/200 - 1/2000. |
IHC-Fr
1/200 - 1/2000. |
In situ hybridization
Use at an assay dependent dilution. |
Target
-
Relevance
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
Alternative names
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab6215 has been referenced in 1 publication.
- Mukaiyama M et al. Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation. J Biochem N/A:N/A (2020). PubMed: 32428205