Anti-Fluorescein antibody [BT.6A4] (Biotin) (ab6215)

Overview

  • Product nameAnti-Fluorescein antibody [BT.6A4] (Biotin)
    See all Fluorescein primary antibodies
  • Description
    Mouse monoclonal [BT.6A4] to Fluorescein (Biotin)
  • ConjugationBiotin
  • SpecificityThe antibody reacts with free and bound fluorescein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purification notesPurified anti-fluorescein biotin conjugate.
  • ClonalityMonoclonal
  • Clone numberBT.6A4
  • Myelomaunknown
  • IsotypeIgM
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000.
IHC-Fr 1/200 - 1/2000.
In situ hybridization Use at an assay dependent dilution.

Target

  • RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

References for Anti-Fluorescein antibody [BT.6A4] (Biotin) (ab6215)

ab6215 has not yet been referenced specifically in any publications.

Product Wall

You could detect the biotinylated antibody directly or use a secondary to the fluorescein antibody.

This antibody has not been tested in flow cytometry. However, in theory it should work in your application.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"