• Product nameAnti-Fluorescein antibody (HRP)
    See all Fluorescein primary antibodies
  • Description
    Rabbit polyclonal to Fluorescein (HRP)
  • ConjugationHRP
  • SpecificitySpecific to Fluoresceine.
  • Tested applicationsSuitable for: IP, IHC-P, WB, ELISAmore details
  • Species reactivity
    No reactivity with immunoglobulin of any species.
  • Immunogen

    Fluorescein coupled to KLH


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA
  • Concentration information loading...
  • Purification notesSolid phase hapten antibody capture. Affinity purified and absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated substitution ratio: HRP:IgG is >1.5
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab19492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 19706527
IHC-P Use at an assay dependent concentration.
WB 1/20 - 1/200.
ELISA 1/200 - 1/1000.


  • RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Anti-Fluorescein antibody (HRP) images

  • Immunohistochemical analysis of chicken trachea tissue, infected with an avian influenza virus, staining Fluorescein with ab19492.
    Fluoroscein-labeled influenza viruses were incubated with tissue overnight at 4°C. Sections were incubated with primary antibody and the signal was amplified with a tyramide signal amplification system.

References for Anti-Fluorescein antibody (HRP) (ab19492)

This product has been referenced in:
  • Kraaij MD  et al. The NOX2-mediated ROS producing capacity of recipient cells is associated with reduced T cell infiltrate in an experimental model of chronic renal allograft inflammation. Transpl Immunol 30:65-70 (2014). Read more (PubMed: 24365514) »
  • Jourdain E  et al. The Pattern of Influenza Virus Attachment Varies among Wild Bird Species. PLoS One 6:e24155 (2011). IHC-P . Read more (PubMed: 21909418) »

See all 4 Publications for this product

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Without a direct comparison, it is a little difficult to choose between the two. The ab6656 datasheet also has a review of IHC with TSA amplification on frozen tissue, after applying the antibody at a dilution of...

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